上个学期的实验室学术讨论会得到了很多老师和同学的支持，在此表示感谢！

本学期第一届学术讨论会将于9月27号召开，王师教授邀请到香港浸会大学的孙进博士来为大家做报告，具体如下：

报告人：孙进博士，香港浸会大学

报告时间：2012.09.27，12:30-13:30

报告题目：Transcriptome-aided proteomic analysis of growth anomalies in the brain corals Platygyra carnosus

附摘要：

Coral growth anomalies (GAs) can reduce the fecundity and survival of reef building corals. To better understand the molecular mechanisms of the development GAs in the structure forming coral Platygyra caronsus, we conducted a de novo sequencing of the transcriptome of this species and used this transcriptome database as a reference to compare the proteome of tissues showing GAs and normal tissues. We sequenced a pooled sample of the normal and GAs tissues using the Illumina “paired-end” sequencing technology and conducted a de novo assembly of the transcriptome to generate a genomic database, and used this database as a reference to support comparative proteomics of the normal tissues and tissues showing GAs using the iTRAQ quantitative proteomic method. The de novo assembly resulted in 162,468 unigenes with an average length of 606 bp (range: 201 to 23,923 bp). BLASTx search against the NCBI protein database showed that 55,355 of the unigenes matched at least a sequence with an E-value of < 10-5, and 59% of the matched sequences were from Metazoa indicating coral origin. We then applied iTRAQ to compare the proteome of GAs tissues and their surrounding normal tissues. A total of 613 coral proteins and 58 putative Symbiodinium proteins were simultaneously quantified in four pairs of coral normal and GAs tissues. Among them, 40 coral proteins and 7 Symbiodinium proteins showed significant differential expression. Two gene ontologies, i.e. proteasome core complex and unfolded protein binding, were enriched in the differentially expressed coral proteins. Protein apoptosis-inducing factor 1 was significantly down-regulated in GAs tissues, indicating that apoptosis was retarded, which probably resulted in larger polys than normal ones. A protein named osteoclast-stimulating factor which can induce bone resorption was significantly up-regulated in GAs tissues, proving an explanation to the abnormal morphology in GAs affected coral skeleton. These preliminary transcriptome aided proteomic analyses have provided candidate proteins for function characterization, which may shed some light on the molecular mechanisms of GAs formation.