秦贞奎 副教授 硕士生导师

电话：0532-82032780

邮箱：qinzk@ouc.edu.cn

地址：鱼山路5号中国海洋大学化学馆326

学习工作经历：

2018.01 - 至今，中国海洋大学，海洋生命学院，副教授

2016.01-2017.12，中国海洋大学，海洋生命学院，讲师

2011.08-2015.12，美国Auburn University，Fisheries and Allied Aquaculture，博士

2008.09-2011.06，中国海洋大学，海洋生物学，硕士

2004.09-2008.06，中国海洋大学，生物科学，学士 

研究领域简介：

主要研究方向为海洋动物生殖繁育及胚胎发育的分子机制，以栉孔扇贝和侏儒蛤等动物为研究对象，通过细胞遗传发育生物学等多种技术手段对其生殖发育关键基因的功能及调控机制进行研究。

主讲课程：

本科生课程《发育生物学》，《发育生物学实验》

主要研究课题：

1.国家自然科学基金青年基金：栉孔扇贝Klf4基因在性别形成中的调控机制研究（31602139，2017.01-2019.12），主持。

2.山东省自然科学基金青年基金：栉孔扇贝Sox2基因在性别形成中的调控机制研究（ZR2016CQ24，2016.11-2019.06），主持。

3.中国海洋大学-Auburn University联合基金I&II：通过高效转基因技术改变鲶鱼中去饱和酶通路以提高 Omega-3 脂肪酸的合成，（2017.01-2018.12），中方主持。

4.国家重点研发计划蓝色粮仓科技创新重点专项：重要养殖贝类种质创制与规模化制种-扇贝优质、抗逆种质创新与规模化制种（2018YFD0901402，2018.12-2022.12），课题骨干。

5.国家重点研发计划蓝色粮仓科技创新重点专项：水产养殖生物性别和发育的分子基础与调控机制-水产养殖动物新种质的繁殖特性及调控机理（2018YFD0900106，2018.12-2022.12），参与。

6.山东省自然科学基金重大基础研究项目：海洋贝类模式动物平台创建（ZR2018ZA0748,2018.09-2020.09），参与。

7.中国海洋大学专项基金：海洋贝类模式动物平台搭建及组学分析（201762001，2017.05-2020.05），参与。 

代表性论文：

1. Jing Wang^†, Lingling Zhang^†, Shanshan Lian^†, ZhenkuiQin^†, et al. Evolutionary transcriptomics of metazoan biphasic life cycle supports a single intercalation origin of metazoan larvae. Nature Ecology & Evolution, 2020, 4: 725–736.

2. Xitan Hou, Maokai Wei, Qi Li, Tingting Zhang, Di Zhou, Dexu Kong, Yueyang Xie, Zhenkui Qin*, Zhifeng Zhang*. Transcriptome analysis on larval segment formation and secondary loss in the echiuran worm Urechis unicinctus. International Journal of Molecular Sciences, 2019, 20:1806.

3. Zhenkui Qin^†, Xueyu Li^†, Danwen Liu, Qing Wang, Li Lu, Zhifeng Zhang*. Analysis of chromosome karyotype and genome size in echiuran Urechis unicinctus Drasche, 1880 (Polychaeta, Urechidae). Comparative Cytogenetics, 2019, 13: 75-85.

4. Shaoshuai Liang, Danwen Liu, Xixi Li, Maokai Wei, Xiaohan Yu, Qi Li, Huixin Ma, Zhifeng Zhang*, Zhenkui Qin*. SOX2 participates in spermatogenesis of Zhikong scallop Chlamys farreri. Scientific Reports, 2019, 9: 76.

5. Zhenkui Qin^†, Yanjie Zhang^†, Huawei Mu, Zhifeng Zhang*, Jian-Wen Qiu*. The sperm proteome of the echiuran Urechis unicinctus (Annelida, Echiura). Proteomics, 2018, 18: 1800107.

6. Yajiao Bai, Di Zhou, Maokai Wei, Yueyang Xie, Beibei Gao, Zhenkui Qin*, Zhifeng Zhang*. Identification of Reference Genes for Normalizing Quantitative Real-Time PCR in Urechis unicinctus. Journal of Ocean University of China, 2018, 17: 614-622.

7. Yang Dandan, Shaoshuai Liang, Qiankun Yang, Danwen Liu, Zhenkui Qin*, Zhang Zhifeng*. Expression characteristics and functional analysis of Krüppel-like factor 4 in adductor muscle and mantle of Zhikong scallop Chlamys farreri. Development Genes and Evolution. 2018, 1-9.

8. Yang Dandan, Zhang Zhifeng, Shaoshuai Liang, Qiankun Yang, Yingrui Wang, Zhenkui Qin*. A novel role of Krüppel-like factor 4 in Zhikong scallop Chlamys farreri during spermatogenesis. PLoS ONE. 2017. 12: e0180351.

9. Ma Xiaoshi^†, Aichang Ji^†, Zhifeng Zhang, Dandan Yang, Shaoshuai, Liang, Yunhan Wang and Zhenkui Qin*. Piwi1 is essential for gametogenesis in mollusk Chlamys farreri.Peer J. 2017. 5: e3412.

10. Shaoshuai Liang, Zhifeng Zhang, Dandan Yang, Yingying Chen, Zhenkui Qin*. Different expression of sox17 gene during gametogenesis between scallop Chlamys farreri and vertebrates. Gene Expression Patterns. 2017. 25-26: 102-108.

11. Zhenkui Qin, Yun Li, Baofeng Su, Qi Cheng, Zhi Ye, D. A. Perera, M. Fobes, Mei Shang, R. A. Dunham*. Editing of the Luteinizing Hormone Gene to Sterilize Channel Catfish, Ictalurus punctatus, Using a Modified Zinc Finger Nuclease Technology with Electroporation. Marine Biotechnology. 2016. 18: 255-263.

12. Xiaoyu Ma, Xiaolong Liu, Di Zhou, Yajiao Bai, Beibei Gao, Zhifeng Zhang*, Zhenkui Qin*. The NF- kB pathway participates in the response to sulfide stress in Urechis unicinctus. Fish & Shellfish Immunology. 2016. 58: 229-238.

13. Zhenkui Qin, Yun Li, Dapeng Sun, Mingyu Shao, Zhifeng Zhang*. Cloning and expression analysis of the vitellogenin gene in the scallop Chlamys farreri and the effects of estradiol-17β on its synthesis. Invertebrate Biology, 2012, 131: 312-321.

14. 梁少帅, 于潇含, 杨丹丹, 秦贞奎^*. 栉孔扇贝sox9基因的 cDNA 克隆及其在不同发育时期性腺中的表达特征. 中国水产科学, 2017, 24: 1184-1192.

15. 李金龙, 秦贞奎, 史晓丽, 张志峰*. 铜离子对单环刺螠的毒性及对体壁抗氧化酶活性的影响. 海洋湖沼通报, 2012, (2): 77-82.

16. 秦贞奎, 刘建国, 许姗姗, 邵明瑜, 张志峰*. 栉孔扇贝核糖体蛋白L24全长cDNA序列的克隆及其性腺表达. 中国海洋大学学报, 2011, 41 (sup.,): 203-207.

参与著作：

1. Rex A. Dunham, Ahmed Elaswad, Zhenkui Qin. Gene Editing in Channel Catfish via Double Electroporation of Zinc-Finger Nucleases. In: Liu J. (eds) Zinc Finger Proteins. Methods in Molecular Biology, vol 1867. Humana Press, New York, NY. 2018: 201-214.

2. Yun Li, Shikai Liu, Ruijia Wang, Zhenkui Qin and Zhangjiang Liu. Bioinformatics Considerations and Approaches for High-Density Linkage Mapping in Aquaculture. In: Liu Z. (eds) Bioinformatics in Aquaculture: Principles and Methods. John Wiley & Sons Ltd, Hoboken, NJ. 2017: 356-379.